Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors

Transaminases have arisen as one of the main biocatalysts for amine production but despite their many advantages, stability is still a concern widespread application. One reasons instability need to use an excess amino donor when trying synthesise amines with unfavourable equilibria. To circumvent this, recycling systems donor, such acid dehydrogenases or aldolases, proved useful push equilibria while avoiding high concentrations. In this work, we report new alanine dehydrogenase from halotolerant bacteria Halomonas elongata which exhibits excellent different cosolvents, combined well characterised CbFDH system L-alanine amination three model substrates step forward, has been co-immobilised and used in flow success re-used dialysis enclosed aromatic aldehyde.